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These artifacts are particularly visible in images with bright, uniform backgrounds (phase contrast, DIC) and in fluorescence images with medium gray or bright backgrounds In order to restore photometric accuracy and remove the defects, the raw image must be adjusted by an operation known as flat-field correction (Gilliland, 1992; Howell, 1992) Only corrected images are suitable for measuring light amplitudes Although flat-field correction is not required to display or print an image, it is very effective in removing disfiguring faults, so the procedure is widely used for cosmetic purposes as well As shown in Figure 15-4 and described here, the correction procedure requires three frames: A raw frame contains the image signal plus signals from optical defects and electronic and thermal noises A flat-field frame is the image of a smooth, featureless field without the object, and, if possible, is made at the same focus as the raw frame.

and to simplify the factors on the right by exploiting conditional independences. As can be seen from the three attribute example above, conditional independences allow us to cancel some of the attributes appearing in the conditions of the conditional probabilities. In this way the factors refer to fewer conditional probability distributions and thus may be stored more efficiently. Since this type of factorization is based on the chain rule of probability, it is often called chain rule factorization (cf. [Castillo et al. 1997]). The notion of a conditional probability also provides a justification of the reasoning scheme outlined in Section 3.3.2, which can be developed in direct analogy to the relational case (recall from 2 that probabilistic reasoning consists in computing conditional probabilities given some observations). In the first step we have to compute the conditional probabilities of all values of attribute B given the observation that the attribute A has the value aobs . That is, we have to compute b dom(B) : P(B = b | A = aobs ) = P

Flat frames should be bright and utilize the full dynamic range of the camera in order to minimize the noise in the final corrected image If both the raw and flat-field frames are dim and noisy, the final processed frame will be very noisy indeed This situation is particularly relevant to fluorescence imaging Therefore, the exposure time for a flat-field frame might be longer than that used for producing the raw image itself To reduce noise.

Beyond this point you might believe that the data that goes into the warehouse is now as near to perfect as it can be made. This is probably true at the time it is written into the database, but surprising though it may seem, this may not be a suf cient condition. So far, we have only discussed what are essentially problems of multiple input points, i.e. an essentially spatial problem. Databases also suffer from temporal problems: a database can remain unchanged, but the circumstances of how it is populated may not. First of all, there is the issue of referential integrity: if, for example, data item Y inherits values, properties, methods, etc. from data item X, then the justi cation for continuing the existence of Y within the data model, depends on the continued existence of X. A subsidiary company can only exist as such, if the parent company continues to exist, to give one simple example. That is not to say that the subsidiary ceases to trade, it

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Some inventors are blinded by their pride They will insist that a jury is certain to comprehend the merits of their inventions Do not listen to such people While it is true that historical data indicates that juries have somewhat of a pro-patent bias, juries are unpredictable Post-trial discussions with jurors, or studies of so-called mock or shadow juries, consistently show that juries often reach their decisions based on matters considered trivial or even irrelevant by both plaintiff and defendant This is especially true when neither side can present a clearly more sympathetic image than the other and the case is to be decided on purely technical (ie, boring) grounds If one can t entertain the jury or garner their sympathy, one can t count on their support The same applies to judges However well versed in the law, precious few judges ever studied the sciences or engineering.